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Literature summary for 1.8.1.4 extracted from

  • Chiranjivi, A.K.; Prakash, J.; Saha, G.; Chandra, P.; Dubey, V.K.
    Mutational studies on Leishmania donovani dihydrolipoamide dehydrogenase (LdBPK291950.1) indicates that the enzyme may not be classical class-I pyridine nucleotide-disulfide oxidoreductase (2020), Int. J. Biol. Macromol., 164, 2141-2150 .
    View publication on PubMed
Search for more literature for 1.8.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta-gami (DE3) cells Leishmania donovani

Protein Variants

Protein Variants Comment Organism
A48I the mutation decreases the Km for dihydrolipoamide substrate by 3fold compared to the wild type enzyme Leishmania donovani
A54I the mutation increases the Km for dihydrolipoamide substrate by 1fold and NAD+ by 3fold compared to the wild type enzyme Leishmania donovani
C15T the mutation increases the Km for dihydrolipoamide substrate by 5fold and NAD+ by 3fold compared to the wild type enzyme Leishmania donovani
C38G the mutation increases the Km for NAD+ by 9fold without affecting Km for dihydrolipoamide compared to the wild type enzyme Leishmania donovani
Cys38Gly the substitution does not show any change in Km value for dihydrolipoamide compared to the wild type enzyme Leishmania donovani
D49G the mutation decreases the Km for dihydrolipoamide substrate by 2fold compared to the wild type enzyme Leishmania donovani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.43
-
 NAD+ wild type enzyme, at pH 7.5 and 37°C Leishmania donovani
0.98
-
 NAD+ mutant enzyme A48I, at pH 7.5 and 37°C Leishmania donovani
1.15
-
 dihydrolipoamide mutant enzyme A48I, at pH 7.5 and 37°C Leishmania donovani
1.31
-
 NAD+ mutant enzyme A54I, at pH 7.5 and 37°C Leishmania donovani
1.43
-
 NAD+ mutant enzyme C15T, at pH 7.5 and 37°C Leishmania donovani
1.5
-
 NAD+ mutant enzyme D49G, at pH 7.5 and 37°C Leishmania donovani
2.2
-
 dihydrolipoamide mutant enzyme D49G, at pH 7.5 and 37°C Leishmania donovani
3.56
-
 dihydrolipoamide wild type enzyme, at pH 7.5 and 37°C Leishmania donovani
3.9
-
 dihydrolipoamide mutant enzyme C38G, at pH 7.5 and 37°C Leishmania donovani
4.1
-
 NAD+ mutant enzyme C38G, at pH 7.5 and 37°C Leishmania donovani
4.7
-
 dihydrolipoamide mutant enzyme A54I, at pH 7.5 and 37°C Leishmania donovani
18.4
-
 dihydrolipoamide mutant enzyme C15T, at pH 7.5 and 37°C Leishmania donovani

Organism

Organism UniProt Comment Textmining
Leishmania donovani
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Leishmania donovani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrolipoamide + NAD+
-
 Leishmania donovani lipoamide + NADH + H+
-
 r

Subunits

Subunits Comment Organism
? x * 99000, MBP-tagged enzyme, SDS-PAGE Leishmania donovani

Synonyms

Synonyms Comment Organism
dihydrolipoamide dehydrogenase
-
 Leishmania donovani
DLDH
-
 Leishmania donovani

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Leishmania donovani
NAD+
-
 Leishmania donovani